Olympic B3 Science Summer Camp 2016
Biotechnology, Biodiversity and Bioinformatics
June 15th - June 30th
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Schedule:
(Subject to change)
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Date - June
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9am-10am
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10am-11am
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11am-11:30pm
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11:30pm-12:00pm
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12:00pm-2pm
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| 15th
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Putnam and Smith
Orientation, Getting Acquainted Activity
Intro to lab:
(1)Establish rules, and set expectations.
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Smith
Pre-Assessment activities
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Guest: Ranger Kelly Cook
Natural History of Crowder's Mountain - Biogeography
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Lunch Break
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Guests: Ranger Kelly Cook and Mr. Steve Barilovits (Treasurer TACF)
The Chestnut Blight, examples of American and Chinese chesnut branches
All: Preparation notes for field trips - collecting/recording, Pryor Farm activities, team duties.
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June 16th - Field Trip to Pryor Farm, at 72 Will Hill Road, Hendersonville, NC.
Route via US 74 West, I-26 West, and US 64 East.
Please arrive at the parking lot by 7:30am at the latest
Wear proper clothing and bring lots of water, sun screen, and your lunch!
If you are traveling separately, please be sure to check your map and have the cell phone of one of the teachers.
Directions from Olympic High School. Head North on Sandy Porter Rd and take the first right onto Arrowood Rd, in 0.4mi turn Left onto 485 Inner North (to Gastonia). Take exit 10 for I-85 toward Gastonia. After 20 miles take exit 10B to get on I74W towards
Kings MT. After 56miles merge onto I-26W. After 16 miles take exit 49A to merge onto US-64E, toward the Bat Cave. Turn Right onto Gilliam MT Road, then Left onto Sugarloaf MT Road, first Left onto Ledbetter Rd, which dead ends at Bald Rock Rd, where you take a slight Left. Bald Rock more
or less dead-ends - take a Right onto Blythe Stepp Rd, the second left onto Timber Trail and continue onto Will Hill Rd. The destination will be on the right. A Hand-drawn map of the last bit is linked here.
PSisco Directions and from Google Maps GoogleMaps Directions
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June 17th - Crowder's Mt State Park Collecting Trip
Unfortunately this is cancelled, we will reschedule in the fall.
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| 17th
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Weller -
(1) Overview of lab activities
(2)Making Lab solutions - how to read and carry out the instructions
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Weller, Smith, Putnam
Lab Skills : accuracy and precision when pipetting, introduction followed by active practice
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Weller, Smith, Putnam
Lab Skills: mixing and centrifuging solutions, introduction followed by active practice
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Lunch Break
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Putnam
Genetics and Punnet squares(presentation)
Lab activities: using the microscope.
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| 20th
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Weller
(1)First steps of the DNA purification method explained and demonstrated
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Weller, Smith, Putnam
Tissue grinding and incubation
Putnam
(1) Plant Breeding: Meiosis and Variation
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Weller, Smith, Putnam
DNA extraction and centrifugation
Putnam
(2) Computational Activity: Punnett Squares
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Lunch Break
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Weller, Smith, Putnam
Separate phases, precipitate DNA
Weller
Biotech Careers; Ethics example of the day
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June 21st -Lab cancelled - water problems at Olympic HS
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June 22nd -Lab cancelled - water problems at Olympic HS
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June 23-24th 2-day Sequencing Lab Field Trip
Field trip to the Bioinformatics Building on the UNC Charlotte campus
Hosts: Dr. Jennifer Weller and Dr. Cathy Moore
Please arrive at the Olympic parking lot by 8:15am
Wear proper clothing for a research lab, lunch will be provided.
If you are traveling separately, please be
sure to check your map and have the cell phone of one of the teachers.
Directions from Olympic HS to Bioinformatics UNC Charlotte
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| 23rd
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Smith
How to check the quality of your DNA: spectrophotometry, gel electrophoresis, PCR
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Smith, Putnam
Using the Nanodrop spectrophotometer
Weller
PCR overview
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Smith, Putnam
Lab skills- pour and load agarose gels
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Lunch Break
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Smith, Putnam
Image the gels with the GelDoc station, set up first PCR
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| 24th
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Weller
Tour of the UNCC campus
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Weller, Smith, Putnam
Load agarose gels with 800bp IGS-trT chloroplast PCR products
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Dr. Cathy Moore
Tour of the Sequencing library prep room and Illumina sequencer
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Lunch Break
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Weller, Smith, Putnam
Make a digital image of gels
Set up the long PCR reactions
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| 27th
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Weller
(1)We are going to repeat the sample extraction with just one sample so students can carry out the full process.
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Weller, Smith, Putnam
Tissue grinding and incubation
Putnam
(1) Plant Breeding: Meiosis and Variation
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Weller, Smith, Putnam
DNA extraction and centrifugation
Putnam
(2) Plant Genetics Part 2
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Lunch Break
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Weller, Smith, Putnam
Separate phases, precipitate DNA
Weller
Biotech Careers; Ethics example (if time permits)
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| 28th
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Weller
Continuation of DNA purification explained and demonstrated
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Weller, Smith, Putnam
Pellet DNA, resuspend, remove RNA and protein
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Weller
PCR primer design
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Lunch Break
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Weller, Smith, Putnam
Phenol-CHCl3 extractions and ethanol precipications
Weller
Biotech Careers; Ethics examples as time permits
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| 29th
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Weller
PCR product cleanup lab: Ampure bead cleanup, pour agarose gels
Weller, Smith, Putnam
Load and run agarose gels (Dr. Weller will image them and post to the Pictures Web page)
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Weller, Smith, Putnam
Trouble-shooting the amplicon preparation process - poor primer design, strategies for work-arounds.
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Lunch Break
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Weller, Smith, Putnam
PLanning time for posters and demonstrations (to make sure materials are available tomorrow).
Biotech Careers; Ethics example as time permits.
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30th
Student Demonstration Day - 6:30-8:30pm - Please come see our results!!!
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Smith
Plan/create posters of results for parents
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Putnam
Plan stations for demos for parents, prepare boxes of materials to take
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Guest speaker
Working in Biotech
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Lunch Break
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With Guests and Judges: 6:30-8:30pm at Central Steele Creek Presbyterian Church Family Life Center,
Show your family and friends what you have learned, Celebrate and see certificates awarded
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Presentations
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Pictures and Blog
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Extra Reading
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Web Links
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Discussion topics
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Lab Protocols
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