Olympic B3 Science Summer Camp 2016
  Biotechnology, Biodiversity and Bioinformatics
  June 15th - June 30th
Protocols: Lab Methods -Updated Frequently
We will post descriptions of procedures, images that we think will help students recognize equipment and details that are easy to forget, and other aids, such as practice sets for carrying out calculations, tutorials and so on. This page will be updated frequently.

June 17 th

  • Understanding Stock Solutions of Reagents Link
  • Pipetting Small Volumes accurately Link
  • Diluting stocks, vortexing,centrifuging small volumes Link
Comments
The skills include safe lab practices, understanding what the stock solutions are that we make assay mixes out of, pipetting tasks and mixing and centrifugation tips.


June 20 th and 21 st
  • Grinding Leaf tissue and extracting DNA Link
Comments
We will grind up leaf tissue and extract the DNA, purify away carbohydrates, lipids, proteins and RNA and concentrate the samples. this takes 2-3 days

June 22 nd

  • DNA quality control parts 1-2 Link
  • DNA quality control part 3 (short amplicon PCR test ) Link
Comments
The skills include using gel electrophoresis to measure the length, and restriction enzyme digestion + gel electrophoresis to determine whether it contains inhibitors.

June 23 rd and 24th

  • Restriction digestion of nuclear DNA with Ampure XP size selecton Link
  • Full-chloroplast amplification with long-amplicon PCR Link
Comments
The skills include using magnetic beads and selective precipitation to collect DNA of the desired length, and long-PCR to amplify the complete chloroplast genome in 15kbp segments. And lots of gels.

Prep Sheets - these show what type of preparation we do prior to each class, perhaps useful.

  • Week 1 (June 15 and 17) Needs updating Link
  • Week 2 (June 20-24) Link
  • Week 3 (June 27-30)

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