Olympic B3 Science Summer Camp 2014
  Biotechnology, Biodiversity and Bioinformatics

  June 11th - June 26th

   This year we have started a class Facebook page, which is at Requires Login
Camp Experiences

This summer's camp will occur once again at Olympic High School in the science labs.
We are grateful for funding from The Burroughs Wellcome Fund for Education Enhancement, and materials from Greiner Bio-One.


Activities: We will do some sample collecting on two field trips: we will collect leaves from American Chestnuts at Crowders Mt. State Park and from an orchard near Hendersonville. Using those samples, students will be introduced to some of the basic skills used in biotechnology labs, and learn what bioinformatics is and how it is used in biotechnology.

Skills include grinding up a sample and extracting the part of it you want (DNA) which requires micropipetting, making solutions, spectrophotometry, gel electrophoresis, and performing enzymatic reactions. Students will be given lots of opportunities to practice these skills while generating molecular DNA markers that help us tell American Chestnuts from other species used in breeding programs to create blight-resistant varieties.
For some of the students this is a first introduction to the tools of a modern molecular biology labs so lots of practice time is included. We will spend time becoming familiar with good practices and including controls in our experiments.

After our two collecting field trips students will also visit a research lab at UNC Charlotte to see how DNA is used to make sequencing libraries in Dr. Weller's lab.

Other topics:

    • Jobs using biotechnology/bioinformatics skills and what types of higher education program students prepared students for those careers.
    • Many ethical concerns come up when carrying out biotechnology research - we will discuss a number of scenarios and what types of legal rules are in place.

By the end of the course students will be able to correctly follow the directions in a lab protocol (a kind of detailed recipe) that yields purified DNA, determine how much DNA they have and how pure it is, perform PCR (a type of molecular biology assay)using that DNA and interpret the results. Students will be able to present the methods and experiments to an audience.

Since our study organism is the American Chestnut students will also learn about the historical place of the American chestnut tree in the North Carolina biosphere and how its loss due to the blight has changed the forest environment, local economies, and communities.

Camp Objectives:

    This camp provides hands-on experience collecting samples and then using wet-lab and computational methods current in biological research; there will be short lectures, lots of discussions, and various Web material that puts the lab experience in context. Our goal is that students will be empowered to pursue experiments of their own.

    To learn more, come ask us questions on Thursday, June 5th at 6:30pm in the Media Center at Olympic High School.

Field Trips

    • June 12th to 72 Will Hill Road, Hendersonville, NC, via US 74 West, I-26 West, and US 64 East - this is near Asheville, and is about 2 hours from Olympic HS. We will help inoculate young trees with fungal spores, and return in the fall to score them for resistance.
    • June 13th to Crowder's Mountain to collect samples.
    • June 20th to the Bioinformatics Building on the UNC Charlotte campus, to visit Dr. Weller's research lab.
Note: Please check the Schedule page for driving directions if you are arriving separately.

Note: Please check the Lists page to make sure you have the necessary permissions and supplies.

When and Where:

Get Acquainted:
Olympic High School Media Center
June 5th 6:30pm

Regular Camp Sessions:
Olympic High School
June 11th - June 26th
Mon-Fri 9am - 2pm
We are still making arrangements about breakfast and lunch - we will add details once we have some answers.
Room 225 will be open for student computer use prior to 9am.
Meet in 240, at 9am
Wet labs: Room 240, 232
Computational lab: Room 225

Overflow space: 228
Parent's Wrap-Up: June 26th 6pm - 8:30pm
Lab demonstrations presented by the students - The Steele Creek Community Room at the Steele Creek Charlotte Medical Center, 13640 Steelcroft Cr., Charlotte NC 28278. Directions

Instructors:
Dr. Jennifer Weller
jweller2@uncc.edu

Mrs. Jeanne Smith
jeanne.smith@cms.k12,nc.us
704-408-7445 (cell)


Mrs. Erica Putnam
ericaa.putnam@cms.k12.nc.us
Saturday Science during the school year

    • September 20th to 72 Will Hill Road, Hendersonville, NC, via US 74 West, I-26 West, and US 64 East - this is near Asheville, and is about 2 hours from Olympic HS. We will help test our fungus-inoculated trees to see which are resistant.
    • October 4th to Stowe Botanical Gardens.
    • November 1st to the Reitzel Lab in the Biology department, Woodward Building on the UNC Charlotte campus.
    • December 6th to the Song Lab, in the Biology department, Woodward Building on the UNC Charlotte campus.
    • January on hiatus.
    • February 7th to the Weller lab in the Bioinformatics Building on the UNC Charlotte campus, to carry out some high-throughput sequencing.
    • March 14 th to the Shi lab in the Bioinformatics Building on the UNC Charlotte campus.
    • April ? th - we are trying to arrange a visit to the GIS mapping lab on the UNC Charlotte campus.
We are planning additional scientific research days during the school year, on a Saturday each month

Please check the link below to find the dates and sign-up information and expectations.

Schedule
Web Links
Articles and Books
Lab protocols
Saturday Science schedule
Lists (Field Trips, Supplies)
Sponsors and Needs
Pictures and Blog
Vocabulary
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