Olympic B3 Science Summer Camp 2012
Biotechnology, Biodiversity and Bioinformatics
June 11th - June 30th
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Schedule:
(Subject to change)
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Date - June
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9am-10am
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10am-11am
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11am-12:00pm
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12:00pm-12:30pm
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12:30pm-2pm
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| 11th
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Putnam and Smith
Getting Acquainted Activity
Intro to lab:
(1)Establish rules, and set expectations.
(2) Lab teams meet at assigned station.
(3)Lab coats
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Smith
Pre-Assessment activities
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Weller
Plant Biology
The Plant Game (generate some data)
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Lunch Break
Note: You will need to bring your own lunch today.
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Smith
(1)Pipetting skills (2) Group Picture
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| 12th
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Weller -
(1) Using Excel to format Data
(2)Pathogen Biology
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Putnam
(1) Meiosis and Variation
(2) Computational Activity: Punnett Squares
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Smith
Lab Skills- using balances
(1)Weighing volumes (2) Spectrophotometry on diluted solutions
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Lunch Break
Note: You will need to bring your own lunch today.
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Smith
(1)Experimental Design: sampling, labelling, preservation
(2) Continue morning Lab Skill activities
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| 13th
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Weller
(1)Learning Box of Chestnut Material
(2)Graphing Results in Excel
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Ranger Kelly Cooke
Natural History of Crowder's Mountain - Biogeography
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Ranger Kelly Cooke
The Chestnut Blight
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Lunch Break
Note: You will need to bring your own lunch today.
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Smith
(1) Rules for the field trip - lists, times and places (2) Methods for Recording Observations
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June 14th
Please arrive at the parking lot by 8:15am
Wear proper clothing and bring lots of water and your lunch!
If you are arriving on your own, please arrive at the Linwood site by 9:15am.
Directions: Take I 85 South (through Gastonia) to Edgewood Road (Exit 13).Take a left (south) and cross over Highway 74. Continue straight.This will be Archie Whitesides Road.
Continue until it dead ends into Linwood Rd.Turn left. The Linwood Access and parking area will be on the right - just a short distance down Linwood.
Map
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| 15th
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Weller
(1) Using Excel to graph The Plant Game data
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Steve Barilovits
The American Chestnut Foundation - breeding and testing for resistance to two blights
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Steve Barilovits
Practical demonstration of hybrids (2) Smith: spectrophotometry
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Lunch Break
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Smith
Lab skills: dilutions and spectrophotometry
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| 18th
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Weller and Putnam
(1) Explaining the DNA extraction protocol through a virtual lab (2) Chemistry - how selective extractions work
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All
Lab skills - grinding plant tissues
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All
Lab skills- using centrifuges
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Lunch Break
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Smith
Lab Skills- removing cell debris by centrifugation
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| 19th
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Weller
(1)Introduction to algorithms
(2) More graphing with Excel (functions and slopes)
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Putnam
Fragmentation of DNA: restriction enzymes and nucleases
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Smith
Lab: selectively solubilize DNA, add RNAse.
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Lunch Break
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Smith and Putnam
Lab: Column purify the DNA and quantify it
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| 20th
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Weller
How much DNA you purified: using Beer's Law
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Smith
Restriction Enzymes
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Putnam
(1) Lab Skills: Set up restriction digestions.
(2) Bacterial DNA extraction (Group 2)
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Lunch Break
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Smith
(1)Lab Skills: Mechanics of pouring agarose gels
(2) Continue DNA purification
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| 21st
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Weller
Image analysis programs for gel electrophoresis
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Putnam
(1) Basics of Gel Electrophoresis
(2) Metagenomics
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Smith
Lab Skills: Load agarose gels with samples and standards, run over lunch
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Lunch Break
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Smith
Lab Skills: Stain gels and document the results
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June 22nd
Field trip to the North Carolina Research Campus (NCRC) in Kannapolis
Host: Dr. Ann Loraine
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| 24th
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Weller
Analysis of the gel results
Interpretation of DNA fingerprints
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Putnam
The polymerase chain reaction
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Smith
Lab Skills: Setting up Master Mixes for RAPD PCR reactions
Programming the thermocyclers
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Lunch Break
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Putnam
Pour agarose gels and prepare samples for loading
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| 25th
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Weller
Designing PCR primers for specific pieces of DNA
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Putnam
Lab skills: load samples and markers on gels, run gels
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Smith
Lab Skills: Set up a gene-specific PCR reaction
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Lunch Break
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Smith
Lab Skills: stain and document gels - use class time to analyse patterns
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| 26th
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Weller
Scoring different types of molecular markers
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Putnam
Pour and load agarose gels for gene-specific PCR products
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Smith
Methods for inserting DNA into foreign cells
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Lunch Break
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Putnam
Stain, image gels and analyse the gene-specific products
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| 27th
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Weller -
Finding matches to DNA sequence: the BLAST algorithm.
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Putnam
The chemistry of sequencing reactions
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Weller
Making sequencing libraries
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Lunch Break
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Smith
Lab skills: fragment DNA non-enzymatically
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28th
Parents Night - 4:30-5:30pm - Please come see our results!!!
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Weller
Bioethics and the ethical, legal and social implications (ELSI) of the Human Genome projects
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Weller
Ethics scenarios for class discussion
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Putnam
Prepare stations for demos for parents
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Lunch Break
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Smith
Prepare posters of results for parents
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June 29th
Field trip to the UNC Charlotte Campus
Host: Dr. Jennifer Weller
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Presentations
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Lists
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Extra Reading
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Web Links
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Discussion Questions
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Lab Protocols
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