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Schedule:
(This schedule is subject to change)
I will post seminar notes, protocols, articles and Web sites that give more detail and context to our activities.
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Week
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Day
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Topics
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Protocols
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Lectures
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| 1
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June 21st
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The Scientific Method
Keeping a good notebook
The American Chestnut
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Introductory Information Stuff like room numbers, phone numbers, etc.
Presentation material Viewgraphs on the 3 topics.
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| 1
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June 22nd
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Chestnut Leaf collection field trip with Steve Barilovits.
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Meet in Plaza-Midwood prepared to collect and label samples
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Field presentation will be reinforced with links and papers
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| 1
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Jun 23rd
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Pipetting practice
Making Dilutions
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Practice Pipetting weighing the drops measured
Practice pipetting Making serial dilutions
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Pipettor parts Also guidelines for good use.
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| 1
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June 24th
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Using the spectrophotometer
Pouring an agarose gel
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Checklist for Nanodrop 1000 use
Directions and advice for setting up gels
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Spectrum Viewgraphs Showing basis of instruments and absorbance vs fluorescence
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| 1
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June 25th
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Calculations, Loading and running an agarose gel of DNA oligomers
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June 28th
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How to do a BLAST analysis of a sequence.
Start DNA purification from leaves
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Plant DNA protocol
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BLAST tutorialChris Overall would like to acknowledge
the great tutorial by Dr. Sandra Porter, that guided this presentation
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| 2
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June 29th
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Complete DNA purification, Quality Control (spectrophotometer and gels)
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No formal seminar - lab day only
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| 2
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June 30th
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Run gels of DNA
Set up PCR reactions
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PCR protocol for ISSR and RAPDs in chestnut
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The paper from which I got the PCR marker information
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| 2
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July 1st
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Run acrylamide gel of PCR products
Seminar on PCR
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PAGE gels General directions
PAGE gels Lab specific directions
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Viewgraphs of my Intro to PCR presentation
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| 2
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July 2nd
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DNA fingerprints and Molecular Markers
Analysis of PCR products
Wrap up class, student impressions and suggestions
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This site was designed by Dr. Andrew Carr and is maintained by Dr. Jennifer Weller
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Dr. Jennifer Weller
Updated 06.27.10